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Document Type

Original Study

Abstract

This study investigate the qualitative and quantitative phytochemicals, antioxidant screening, and the reducing effect of intraocular pressure of P. tuber-regium aqueous extract in rabbits. Standard protocols were used to analyze the qualitative and quantitative phytochemical, in-vitro antioxidant studies ferric reducing antioxidant power (FRAP), total antioxidant capacity) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) were also used to assess these plants. Thirty (30) New Zealand male rabbits were randomly selected for this study. The whole group except normal control were induced with 1% Prednisolone acetate suspension for 3 days to obtain the baseline intraocular pressure (IOP) >5-10 mm Hg, afterward treated with a graded oral dose of 100, 200, and 400 mg/kg P. tuber-regium respectively. The application was administered daily in the right eye (OD) only for 7 days, and the IOP measurements were. The phytochemical screening of the extract showed the presence of flavonoids, phenol, tannins, and saponins. Tannins and phenol were abundant (28 and 20 mg/kg). The antioxidant scavenging property showed the potential capacity of P. tuberregium extract to scavenge free radicals against oxidative stress at 10 %, 100 %, and 25.26 % of P. tuber-regium aqueous extract when compared with ascorbic acid. The result from this present study at graded doses (100, 200, and 400 mg/kg) of P. tuberregium aqueous extract elicited a significant decrease in intraocular pressure levels, specifically at 200 and 400 mg/kg of the mushroom extract had a markable reduction in

the mean IOP level when compared with timolol control and untreated control (p

Keywords

Phytochemistry, In-vitro antioxidant, intraocular pressure, Pleurotus tuber-regium

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