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Document Type

Article

Abstract

The study was conducted on 444 patients suffering from skin lesions of different ages and genders that were diagnosed by dermatologists as cutaneous leishmaniasis for people reviewing and throughout the days of the week for the department of epidemiology and transitional diseases who attended to hospitals in the city of Baghdad, namely (Al-Shaheed Dari Al Fayyad Teaching Hospital , Al-Shaheed Al Sadr Hospital , Al-Numan Teaching Hospital, Al-Karama Teaching Hospital) for the study period from October 1, 2019 until the end of March 2020. The methods of molecular diagnostics showed that multiplex pcr examined 40 random samples of the parasite that were collected through this technique from blood samples taken from the ulcers of infected people to reveal the DNA parts of the Leishmania parasite. Special prefixes for the Leishmania parasite species showed that 28 positive samples contain the parasite's DNA and 12 negative samples, of which 11 are for the Leishmania tropica type (27.5%) and 17 samples for the Leishmania major type(42.5%) at the molecular weight 1150 base pair. 30 serum samples were taken from patients diagnosed with cutaneous leishmaniasis for serological tests. The current study showed a significant decrease in immunoglobulin G for patients with cutaneous leishmaniasis by 1205.100 ± 210.155 , The current results also showed a significant decrease in the level of immunoglobulin M in patients with cutaneous leishmaniasis by 90.366 ± 26.181

Keywords

IgG, IgM, Leishmania, Cutaneous, Blood, Antigens, multiplex pcr

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