Isolation And Identification Of The Fungus Monascus Purpureus From Imported Currants As a New Record In Iraq

Ghasaq Malik Al-Barkawie, Department of Biology, College of Science, University of Qadisiyah, Qadisiyah, IraqFollow
A.S. Saadon, Department of Biology, College of Science, University of Qadisiyah, Qadisiyah, Iraq

Document Type

Article

Abstract

This study was conducted in the Laboratory of Fungus in the Department of Biology / College of Science / University of Qadisiyah to isolate and diagnose some insulation from fungi isolated from imported dried fruits (raisins) in Qadisiyah province, Iraq. The isolations were diagnosed both morphologically and microscopically using the classification keys and to confirm the appearance and microscopic diagnosis diagnosed using polymerase chain reaction(PCR), And determine the sequence of nitrogen bases (Nucleotide sequence(of compound DNA products using ITS1 and ITS4. The results of the nucleotide sequence analysis of DNA (PCR product) compounding innate isolation and using BLAST to compare with data available at the U.S. National Center for Biotechnology Information (NCBI) have shown that this isolation belongs to the type Monascus purpureus. By comparing the sequence of nitrogen bases of isolated M. purpureus fungus in this study, it was found that there was a 100% similarity to many of the M. purpureus fungus isolates previously registered at the National Center for Biotechnology Information (NCBI), including those diagnosed in China (MT361825, MK359689, MW581230) and Japan (AB477248).

Keywords

Monascus purpureus, Iraq, imported currants

Recommended Citation

Al-Barkawie, Ghasaq Malik and Saadon, A.S. (2021) "Isolation And Identification Of The Fungus Monascus Purpureus From Imported Currants As a New Record In Iraq," Al-Qadisiyah Journal of Pure Science: Vol. 26 : No. 5 , Article 9.
Available at: https://doi.org/10.29350/qjps.2021.26.5.1444