Evaluation Of The Ability Of Some Bacterial Species Isolated From UTI To Form Biofilm

Dhuha Mahdi Jabir, a College of Science, University of Al-Qadisiyah, P.O.Box.1895, IraqFollow

Document Type

Article

Abstract

Urinary tract infection is one of the most serious infectious diseasesin the world, This study aimed to isolate and diagnose the bacteria that cause UTI and then evaluate its ability to form a biofilm. 100 urine samples were collected for a group of patients attending Al-Diwaniyah Teaching Hospital, who were confirmed to have UTIsat different ages. The results showed the presence of bacteria. Escherichia coliand Proteus sppin all samples(100%), while Staphylococcus aureus was found in 87% of samples, Enterobacter spp was isolated by 67%,and Pseudomonas aeruginosaby 65%.Klebsiella pneumoniaat 50 % and Staphylococcus epidermidisand Micrococcus sppeach with 30%. Regarding the ability of the isolated bacteria to form a biofilm, two methods used Congo-red agar and tube method,and the results were as follows 64% for E.coli, K pneumoniawith 66.6% while S. aureusshowed the ability of formation with 41.6%,and negative results for the rest by using tube method while Congo-red agar method results were 25% for 50% for E.coli and S. aureus. The study concluded that Congo red agar method is easy to perform and interpret, while the tube method is highly sensitive and specific.

Keywords

UTI, biofilm, Congo red agar, tube method

Recommended Citation

Jabir, Dhuha Mahdi (2022) "Evaluation Of The Ability Of Some Bacterial Species Isolated From UTI To Form Biofilm," Al-Qadisiyah Journal of Pure Science: Vol. 27 : No. 1 , Article 13.
Available at: https://doi.org/10.29350/qjps.2022.27.1.1492